System for measuring oxygen consumption rates of mammalian cells in static culture under hypoxic conditions.
Identifieur interne : 000004 ( Main/Exploration ); précédent : 000003; suivant : 000005System for measuring oxygen consumption rates of mammalian cells in static culture under hypoxic conditions.
Auteurs : Yuki Kagawa [Japon] ; Hirotaka Miyahara [Japon] ; Yuri Ota [Japon] ; Satoshi Tsuneda [Japon]Source :
- Biotechnology progress [ 1520-6033 ] ; 2016.
Abstract
Estimating the oxygen consumption rates (OCRs) of mammalian cells in hypoxic environments is essential for designing and developing a three-dimensional (3-D) cell culture system. However, OCR measurements under hypoxic conditions are infrequently reported in the literature. Here, we developed a system for measuring OCRs at low oxygen levels. The system injects nitrogen gas into the environment and measures the oxygen concentration by an optical oxygen microsensor that consumes no oxygen. The developed system was applied to HepG2 cells in static culture. Specifically, we measured the spatial profiles of the local dissolved oxygen concentration in the medium, then estimated the OCRs of the cells. The OCRs, and also the pericellular oxygen concentrations, decreased nonlinearly as the oxygen partial pressure in the environment decreased from 19% to 1%. The OCRs also depended on the culture period and the matrix used for coating the dish surface. Using this system, we can precisely estimate the OCRs of various cell types under environments that mimic 3-D culture conditions, contributing crucial data for an efficient 3-D culture system design. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:189-197, 2016.
DOI: 10.1002/btpr.2202
PubMed: 26558344
Affiliations:
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Shinjuku, Tokyo, 162-8480, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Dept. of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480</wicri:regionArea><wicri:noRegion>162-8480</wicri:noRegion></affiliation></author><author><name sortKey="Ota, Yuri" sort="Ota, Yuri" uniqKey="Ota Y" first="Yuri" last="Ota">Yuri Ota</name><affiliation wicri:level="1"><nlm:affiliation>Dept. of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Dept. of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480</wicri:regionArea><wicri:noRegion>162-8480</wicri:noRegion></affiliation></author><author><name sortKey="Tsuneda, Satoshi" sort="Tsuneda, Satoshi" uniqKey="Tsuneda S" first="Satoshi" last="Tsuneda">Satoshi Tsuneda</name><affiliation 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wicri:level="1"><nlm:affiliation>Dept. of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Dept. of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480</wicri:regionArea><wicri:noRegion>162-8480</wicri:noRegion></affiliation></author><author><name sortKey="Ota, Yuri" sort="Ota, Yuri" uniqKey="Ota Y" first="Yuri" last="Ota">Yuri Ota</name><affiliation wicri:level="1"><nlm:affiliation>Dept. of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Dept. of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480</wicri:regionArea><wicri:noRegion>162-8480</wicri:noRegion></affiliation></author><author><name sortKey="Tsuneda, Satoshi" sort="Tsuneda, Satoshi" uniqKey="Tsuneda S" first="Satoshi" last="Tsuneda">Satoshi Tsuneda</name><affiliation wicri:level="1"><nlm:affiliation>Inst. for Nanoscience and Nanotechnology, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Inst. for Nanoscience and Nanotechnology, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku, Tokyo, 162-8480</wicri:regionArea><wicri:noRegion>162-8480</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biotechnology progress</title><idno type="eISSN">1520-6033</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Estimating the oxygen consumption rates (OCRs) of mammalian cells in hypoxic environments is essential for designing and developing a three-dimensional (3-D) cell culture system. However, OCR measurements under hypoxic conditions are infrequently reported in the literature. Here, we developed a system for measuring OCRs at low oxygen levels. The system injects nitrogen gas into the environment and measures the oxygen concentration by an optical oxygen microsensor that consumes no oxygen. The developed system was applied to HepG2 cells in static culture. Specifically, we measured the spatial profiles of the local dissolved oxygen concentration in the medium, then estimated the OCRs of the cells. The OCRs, and also the pericellular oxygen concentrations, decreased nonlinearly as the oxygen partial pressure in the environment decreased from 19% to 1%. The OCRs also depended on the culture period and the matrix used for coating the dish surface. Using this system, we can precisely estimate the OCRs of various cell types under environments that mimic 3-D culture conditions, contributing crucial data for an efficient 3-D culture system design. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:189-197, 2016.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Kagawa, Yuki" sort="Kagawa, Yuki" uniqKey="Kagawa Y" first="Yuki" last="Kagawa">Yuki Kagawa</name></noRegion><name sortKey="Miyahara, Hirotaka" sort="Miyahara, Hirotaka" uniqKey="Miyahara H" first="Hirotaka" last="Miyahara">Hirotaka Miyahara</name><name sortKey="Ota, Yuri" sort="Ota, Yuri" uniqKey="Ota Y" first="Yuri" last="Ota">Yuri Ota</name><name sortKey="Tsuneda, Satoshi" sort="Tsuneda, Satoshi" uniqKey="Tsuneda S" first="Satoshi" last="Tsuneda">Satoshi Tsuneda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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